Services

Natural Remedies Pvt. Ltd. offers contractual research & analytical services in the areas like phytochemistry, in-vitro bioassays, experimental pharmacology, microbiology, qualitative and quantitative analysis, phytochemical and residual analysis. Our scientists apply cutting-edge technology and ideas to your research programs and deliver a flexible, tailored approach to all research objectives, whether a one-off service or a complete research program.

In addition to providing the traditional values of reproducibility, accuracy, efficient timelines and quality reporting, our contractual services are characterized with:

  • Custom study design and data interpretation by highly experienced scientists
  • Collaborative approach during the scientific process to meet the study objectives
  • Capability to develop new models to fulfill unmet needs

The R&D centre is spread over 25000 sq ft. and employs over 100 scientists from different disciplines. It performs various pre-clinical studies under a prestigious system of GLP. Natural Remedies R&D Centre received GLP certificate from National Good Laboratory Practice (GLP) Compliance Monitoring Authority (NGCMA) for conducting nonclinical studies for herbs and others. The R&D centre is accredited by NABL (National Accreditation Board for Testing and Calibration Laboratories) as per ISO/IEC.17025:2005 for biological and chemical testing.  Our analytical chemistry department is also a Government approved Public Testing laboratory for analysis of Ayurveda, Siddha and Unani ingredients, Microbiology and finished formulations.

Natural Remedies Pvt. Ltd., is a recognized leader in the field of Herbs and Phytocompounds, as proven by accolades received from organizations and personnel from across the world.

The analytical laboratory has contributed many monographs on Indian medicinal plants to various National and International Pharmacopoeia.

In-vitro Assay (Cell assay & Cell-free assay): Standardized/custom programs of preclinical discovery solutions to meet the needs of the Pharmaceutical, Natural Product and Biotechnology Industries. Biochemical/Enzyme inhibition assays are biological methods through which the bioactivity of test substances can be assessed. In the preclinical discovery market, the versatility of enzyme-based assays, a lesser turnaround time and low costs influence most companies to choose enzyme based assays over others. This unit has standardized and routinely perform more than 70 validated in-vitro assays targeting various therapeutic areas using specialized assay technologies based on different detection models like absorbance (Colorimetric, ELISA), Luminescence and Fluorescence (Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence) in 96 and 384 well formats. We provide a spectrum of these services in cellular assays that are designed to accelerate the pace and increase the productivity of discovery and development programs. Screening and assay development remains one of the most challenging and resource-intensive steps in the drug discovery process, with the end result depending on the proper balance of assays, experimental design, and data management. Natural Remedies provides a broad spectrum of high-quality service to support various stages of drug discovery and development. With state-of-the-art facilities and instrumentation, we offer services in assay development, compound profiling and in vitro safety. Natural Remedies uses a number of primary and immortalized cell lines to assay test compounds. We are committed to providing high quality, cost-effective in vitro services with a rapid turnaround time.

Using these in vitro cell based assays, compounds can be rapidly and cost effectively screened for activity prior to more expensive and time-consuming in vivo analysis.

Safety assessment is an important criterion for compound development before expensive preclinical and clinical studies. In order to predict Genotoxicity or specific cellular impacts, Natural Remedies provides GLP/Non-GLP in vitro genetic testing as per OECD guidelines (Mouse Lymphoma, Ames, Chromosome Aberration, Micronucleus, Steroidogenesis Assay, in-vitro Skin corrosion, Eye Irritation, Skin irritation). All work is carried out under well-defined standard operating procedures (SOPs) by highly qualified scientists.

ADME assay: As researchers try to predict the absorption, distribution, metabolism and excretion (ADME) characteristics of a compound, the advent of various in-vitro high throughput assays has come as a boon. The liver is the central organ in ADME. It is responsible for most of the metabolic processes involving the breakdown of drugs and their excretion from the body. The cytochrome P450 superfamily of enzymes sits at the crossroads of liver metabolism, encompassing dozens of gene products that catalyze the breakdown of drugs and toxins. We at Natural Remedies have standardized several Cytochrome P450 assays that are useful for studying interaction as well as metabolism. In addition, we have standardized various in-vitro permeability models like PAMPA and Caco-2 monolayer permeability assay that are useful for absorption studies.

For more information please visit www.bio-opt.com write to bioopt@naturalremedy.com

Specially for Natural Product

Contractual Services:

Bioactivity Guided Fractionation

Bioactivity guided fractionation & isolation of active principles:

Contractual Research, a wing of Natural Remedies is now offering, a service to isolate and identify bioactive constituents from herbal products. This new service has been the result of effective combination of Natural Remedies traditional strengths in phytochemistry with the battery of in-vitro bioassays (biochemical and cell based) standardized in-house.
Natural Remedies has taken up the initiative in isolating, purifying & characterizing over 200 different phytochemical constituents from important Indian medicinal plants to use as Primary Phytochemical Reference substance (www.phytocompounds.com). These reference substances have been characterized using chromatographic (TLC / HPTLC / HPLC), spectroscopic (UV, FTIR, NMR & Mass) and physico-chemical methods assuring their identity & purity.

Typical steps involved in Bioactivity guided fractionation:

  • Finalization of the bioassay based on the end use of the product (for example, DPP IV inhibition assay for healthy blood sugar claims)
  • Fractionation of the extract / product which will be guided by bioassay
  • Isolation of bioactive constituent(s) from the active fractions
  • Characterization of bioactive constituent(s) by spectroscopic (NMR, IR etc,.) methods
  • Chromatographic method (HPLC/HPTLC etc.,) for estimation of bioactive constituents in the product

What do you get from Bioactivity guided fractionation ?

  • Identification of bioactive constituent(s) of the herbs being used in your product
  • Reference standard of the bioactive constituent(s) of your product
  • Chromatographic method for estimation of bioactive constituent(s) in your product (can be used for meaningful quality control)

Natural remedies Bioassay Services

Biochemical assay:

Standardized / custom programs of preclinical discovery solutions to meet the needs of the Pharmaceutical, Natural Product and Biotechnology Industries. We have standardized and routinely perform more than 50 in-vitro assays relevant to selection and optimization of new drug candidates using specialized assay technologies based on different detection modes like Absorbance (colorimetric, ELISA), Luminescence and Fluorescence (Fluorescence Intensity, Fluorescence Polarization, Time Resolved Fluorescence etc) in 96 and 384 well formats. We provide a spectrum of these services in cellular assays that are designed to accelerate the pace and increase the productivity of discovery and development programs. We are committed in providing high quality, cost effective in vitro services with a rapid turnaround time. Apart from new product development, cell assays can be effectively be used for screening campaigns, bio-activity guided fractionation, compatibility studies, stability studies and for comparing various prototypes/formulations. In-vitro efficacy studies helps to screen the drug for its various therapeutic applications using high throughput model, thereby cost effective, repoducible results can be generated. In-vitro preclinical safety studies are standardized according to OECD guideline. These assays facilitates to explore the genotoxic potential of molecule.

Sl.No. code Bioassay Pharmacological Relevance
1 BCA01 α - Amylase inhibition Obesity, Type II Diabetes, Carbohydrate blocker
2 BCA02 α - Glucosidase inhibition Type II Diabetes, Carbohydrate blocker
3 BCA03 β - Glucuronidase inhibition Hepatoprotection, Axillary odour
4 BCA04 ABTS radical scavenging Anti-oxidant
5 BCA05 Acetylcholinesterase inhibition Cognition enhancement, Neuro- degenerative disorders
6 BCA06 Aryl Sulphatase inhibition Axillary odour
7 BCA07 Butyrylcholinesterase inhibition Neuro- degenerative disorders
8 BCA08 Collagenase  inhibition Antiageing / Antiwrinkle
9 BCA09 Nitric-oxide scavenging Anti-oxidant, Alzheimer’s disease
10 BCA10 Superoxide anion scavenging (PMS-NADH system) Anti-oxidant
11 BCA11 DPP IV inhibition Wrinkle, Ageing
12 BCA12 DPPH radical scavenging Inflammation, Anti-allergy
13 BCA13 Elastase inhibition Anti-oxidant
14 BCA14 Hyaluronidase inhibition Inflammation, Anti-allergy
15 BCA15 Hydroxyl radical scavenging Obesity
16 BCA16 Lipase inhibition Obesity
17 BCA17 15 - Lipoxygenase  inhibition Inflammation
18 BCA18 Trypsin inhibition Inflammation
19 BCA19 Xanthine oxidase inhibition Gout
20 BCA20 TC- PTPase inhibition Selectivity testing assay for anti-phosphatase activity

Cell assay:

Screening and assay development remains one of the most challenging and resource-intensive steps in the drug discovery process, with the end result depending on proper balance of assays, experimental design, and data management. Natural Remedies provides a broad spectrum of high quality service to support various stages of drug discovery and development. With state-of-the-art facilities and instrumentation, we offer services in assay development, compound profiling and in vitro safety. Natural Remedies uses a number of primary and immortalized cell lines to assay test compounds. We have 50 validated in vitro assays targeting various therapeutic areas using specialized assay technologies based on different detection models like absorbance (Colorimetric,ELISA), Luminescence and Fluorescence (Fluorescence Intensity, Fluorescence Polarization, Time Resolved Fluorescence) in 96 and 384 well formats. Using these in vitro cell based assays, compounds can be rapidly and cost effectively screened for activity prior to more expensive and time consuming in vivo analysis.

Safety assessment is an important criterion for compound development before expensive preclinical and clinical studies. In order to predict Genotoxicity or specific cellular impacts, Natural Remedies provides in vitro genetic testing (Ames, Chromosome Aberration, Micronucleus).

All work is carried out under well-defined standard operating procedures (SOPs) by highly qualified scientists. Most studies can be conducted under Good Laboratory Practice (GLP) compliance if required.

Sl.No. code Bioassay Pharmacological Relevance
1 CA01 Lymphocyte proliferation
a. Naïve and LPS treated lymphocytes
b. Naïve and PHA induced
c. Naïve and Con A treated lymphocytes
d. Naïve and PWA induced
e. Naïve and anti-CD3 induced
Immune modulation
2 CA02 NO release Immune stimulation
3 CA03 NO scavenging Anti-oxidant, Inflammation, Pain
4 CA04 Macrophage phagocytosis Immune modulation
5 CA05 LTB4 release Inflammation, Pain
6 CA06 Hepatoprotection using t-BH Liver health
7 CA08 Histamine release Inflammation, Allergy
8 CA09 Chromosomal aberration assay Safety
9 CA10 Micronucleus assay Safety
10 CA11 Melanin release Skin Tanning / Lightning
11 CA12 PGE2 release Inflammation, Pain
12 CA13 Insulin release Diabetes
13 CA14 Collagen release Wrinkle, Ageing
14 CA15 TXB2 release Inflammation, Allergy, Pain
15 CA16 IL-1 beta inhibition Inflammation, Allergy
16 CA17 TNF alpha inhibition Inflammation, Allergy
17 CA18 AMES test Safety evaluation
18 CA19 IL-2 release (Con A induced) Immune modulation
19 CA20 IL-4 release (Con A induced) Immune modulation
20 CA21 IL-6 release (Con A induced) Immune modulation
21 CA22 IFN gamma release (Con A induced) Immune modulation
22 CA23 Neutral Red Uptake assay CC50 correlation to acute oral rodent LD50, safety evaluation
23 CA24 Cellular antioxidant assay Psoriasis
24 CA25 Hepatoprotection against Diazinon induced hepatotoxicity Liver health
25 CA36 IL-6 inhibition Inflammation
26 CA27 IL-6 release (PHA induced) Immune modulation
27 CA28 IL-6 release (PHA induced) Immune modulation
28 CA29 IL-2 release (PWA induced) Inflammation
29 CA30 IL-6 release (PWA induced) Immune modulation
30 CA31 IL-2 release (Anti CD3 induced) Immune modulation
31 CA32 IKK-β (NF-B kinase) inhibition IL-6 release (Anti CD3 induced)
32 CA33 IL-6 release (LPS induced) Immune modulation
33 CA34 IL-12 release (LPS induced) Immune modulation
34 CA35 Hepatoprotection assay against Aldrin (OC) Liver health
35 CA36 Adipogenesis assay Obesity
36 CA37 IL-3 release (Con A induced) Immune modulation
37 CA38 IL-5 release Con A induced) Immune modulation
38 CA39 IL-10 release (Con A induced) Immune modulation, Inflammation
39 CA40 IL-10 release (LPS induced) Immune modulation, Inflammation
40 CA41 Hepatoprotection against Aflatoxin(AFB1) induced hepatotoxicity in Human HepG2 cells Liver health
41 CA42 Hepatoprotection against Galactosamine induced hepatotoxicity in Human HepG2 cells Liver health
42 CA43 Adipolysis Obesity
43 CA44 MCP-1 (Monocytic chemotactic protein) inhibition Inflammation
44 CA45 MCF-7 anti-proliferative assay Cancer

List of Publications:

  • Modulation of lipopolysaccharide-induced pro-inflammatory mediators by an extract of Glycyrrhiza glabra and its phytoconstituents. Thiyagarajan P, Chandrasekaran CV, Deepak HB, Agarwal A. Inflammopharmacology. 2011. PMID: 21328091.

  • Optimization of cell-based assays to quantify the anti-inflammatory/allergic potential of test substances in 96-well format. Chandrasekaran CV, Edwin Jothie R, Kapoor P, Gupta A, Agarwal A. Inflammopharmacology. 2011 Jun;19(3):169-81. PMID: 21069571.

  • In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract. Chandrasekaran CV, Thiyagarajan P, Deepak HB, Agarwal A. Int Immunopharmacol. 2011 Jan;11(1):79-84. PMID: 21034865.
  • Dual inhibitory effect of Glycyrrhiza glabra (GutGard™) on COX and LOX products. Chandrasekaran CV, Deepak HB, Thiyagarajan P, Kathiresan S, Sangli GK, Deepak M, Agarwal A. Phytomedicine. 2011 Feb 15;18(4):278-84. PMID: 20864324.

  • Effect of an extract of Andrographis paniculata leaves on inflammatory and allergic mediators in vitro. Chandrasekaran CV, Gupta A, Agarwal A. J Ethnopharmacol. 2010 May 27;129(2):203-7. PMID: 20307638.
  • In vitro efficacy and safety of poly-herbal formulations. Chandrasekaran CV, Sundarajan K, David K, Agarwal A. Toxicol In Vitro. 2010 Apr;24(3):885-97. PMID: 19958825.

  • Tinospora cordifolia, a safety evaluation. Chandrasekaran CV, Mathuram LN, Daivasigamani P, Bhatnagar U. Toxicol In Vitro. 2009 Oct;23(7):1220-6. PMID: 19651204.

  • Evaluation of the genotoxic potential and acute oral toxicity of standardized extract of Andrographis paniculata (KalmCold). Chandrasekaran CV, Thiyagarajan P, Sundarajan K, Goudar KS, Deepak M, Murali B, Allan JJ, Agarwal A. Food Chem Toxicol. 2009 Aug;47(8):1892-902. PMID: 19447157.

ADME assay:

As researchers try to predict the absorption, distribution, metabolism and excretion (ADME) characteristics of a compound, the advent of various in-vitro high throughput assays has come as a boon. The liver is the central organ in ADME. It is responsible for most of the metabolic processes involving break down of drugs and their excretion from the body. The cytochrome P450 superfamily of enzymes sits at the crossroads of liver metabolism, encompassing dozens of gene products that catalyze the breakdown of drugs and toxins. We at Natural Remedies have standardized several Cytochrome P450 assays that are useful for studying interaction as well as metabolism. In addition we have standardized various in-vitro permeability models like PAMPA and Caco-2 monolayer permeability assay that are useful for absorption studies.

Sl.No. code Bioassay Pharmacological Relevance
1 AM01 Cytochrome P450 2A5 (m) inhibition Interaction
2 AM02 Cytochrome P450 2A6 (h) inhibition Interaction
3 AM03 Cytochrome P450 3A4 (H) inhibition Interaction
4 AM04 Cytochrome P450 2D6 (h) inhibition Interaction
5 AM05 Glutathione S-transferase (GST) (r) inhibition Interaction
6 AM06 UDP Glucuronosyltransferase (UGT) (h) inhibition Interaction

Disclaimer

The in-vitro bioassays given in this list / catalogue are being offered under company’s contract research program. In-house standardized assay protocols will be used to perform these assays. The company is committed to ensure that utmost care is taken to adhere to specified protocols towards providing accurate and factual results to the users of this service. However, further use of the results and analytical reports provided by Natural Remedies for any commercial purposes is the sole responsibility of the user and Natural Remedies does not make any representation that the use of the bioassay reports will not infringe any intellectual property rights of third parties. In no event shall Natural Remedies be liable to any party for any direct, indirect, special or other consequential damages for any commercial use of the bioassay reports provided by Natural Remedies.

Analytical laboratory work in state-of-the-art facilities with cutting edge equipment and analytical infrastructure to cater to the demands of Food and Pharmaceutical Industry. This Laboratory offers the analytical services required to meet Pharmacopeial and Food Chemical Codex (FCC) monograph standards. Natural Remedies uses established and standard methodologies as the Association of Analytical Chemists (AOAC), Pharmacopoeial, BIS etc,. In cases where existing methodologies are not sufficient, Natural Remedies has the expertise and resources to develop and validate assays.


Stability studies / Shelf life: Stability studies / Shelf life: Shelf-life refers to the period from initial preparation to packaging during which the drug dosage form continues to remain within its physical, chemical, therapeutic and toxicological specifications at specified storage conditions.The stability of the product is its ability to resist deterioration. It is always expressed in terms of shelf life. Natural Remedies offers stability management services for Active Neutraceutical Ingredients (APIs) and finished products. The facilities are equipped with temperature and humidity-controlled chambers that are continuously monitored and alarmed for any environmental changes. All the chambers undergo stringent IQ, OQ, PQ and calibration processes on a yearly basis and are fully mapped (empty and full) for both temperature and humidity to ensure temperature/humidity uniformity. The chambers meet complete ICH conditions, including 25°C/60% RH, 30°C/65% RH, 40°C/75%RH, 5°C.

  • Real Time Stability Studies and Testing
  • Accelerated Aging Stability Studies and Testing
  • Stress testing

Analytical Services List

# Botanical Name Marker(s) / active compounds
1 Acorus calamus a-asarone
2 Adhatoda vasica Vasicine
3 Aegle marmelos Marmelosin
4 Albizia lebbeck (+)-Catechin
5 Aloe vera Aloin
6 Andrographis paniculata Andrographolide
7 Anethum sowa Anethol
8 Asparagus racemosus Shatavarin-I and Shatavarin-IV
9 Bacopa monnieri Bacoside-A
10 Berberis aristata Berberine
11 Boerhaavia diffusa Eupalitin& Eupalitin-3-O-galactoside
12 Boswellia serrata Acetyl keto boswellic acid, Keto Boswellic acid
13 Capsicum annum Capsaicin
14 Cassia angustifolia Sennosides
15 Cassia tora Chrysophanol
16 Coleus forskohlii Forskolin
17 Commiphora mukul Guggulsterone-E & Z
18 Curcuma longa Curcumin
19 Eclipta alba Wedelolactone
20 Gardenia gummifera Gardenin
21 Gloriosa superba Colchicine
22 Glycyrrhiza glabra Glycyrrhizin
23 Gymnema sylvestre Gymnemagenin
24 Largerstroemia speciosa Corosolic acid
25 Mallotus philippensis Rottlerin
26 Mangifera indica Mangiferin
27 Mangifera indica Mangiferin
28 Mucuna pruriens L-Dopa
29 Ocimum tenuiflorum Rosemarinic acid, Ursolic acid
30 Peganum harmala Harmaline, Harmine
31 Phyllanthus amarus Phyllanthin, Hypophyllanthin
32 Piper longum / Piper nigrum Piperine
33 Plumbago zeylanica Plumbagin
34 Psoralea corylifolia Psoralen
35 Terminalia arjuna Arjungenin, Arjunic acid
36 Tribulus terrestris Tribulosin
37 Trigonella-foenum-graecum Trigonelline
38 Valeriania officinalis Valerenic acid
39 Vitex negundo Agnuside
40 Withania somnifera Withanolide-A & B, Withastramonolide, Withanoside- IV & V, Withaferin-A
41 Zingiber officinale 6-gingerol, 6-Shogaol, 8-gingerol
# Botanical Name Marker(s) / active compounds
1 Acacia catechu Catechins
2 Adhatoda vasica Vasicine
3 Aegle marmelos Marmelosin
4 Aesculus indica b-escin
5 Andrographis paniculata Andrographolide
6 Aristolochia indica Aristolochic acid
7 Bacopa monnieri Bacoside A, Bacopaside I, Bacosine
8 Bacopa monnieri Bacoside-A
9 Berberis aristata Berberine
10 Boswellia serrata Boswellic acids
12 Camellia sinensis EGCG (Epigalocatechin gallate) and Total Catechins
13 Cassia angustifolia Sennosides A & B
14 Cassia tora Chrysophanol
15 Capsicum annum Capsaicin
16 Centella asiatica Asiaticoside and Madecassoside
17 Coleus forskohlii Forskolin
18 Chrysanthemum parthenium Parthenolide
19 Curcuma longa Curcumin
20 Eclipta alba Wedelolactone & demethylwedelolactone
21 Embelia ribes Embelin
22 Ferula asafoetida Ferulic acid
23 Garcinia cambogia Hydroxy citric acid
24 Garcinia mangostana Mangostin
25 Gardenia gummifera Gardenin
26 Gloriosa superba Colchicine
27 Glycyrrhiza glabra Glabridin
28 Glycyrrhiza glabra Glycyrrhizin
29 Gymnema sylvestre Gymnemagenin
30 Hypericum perforatum Hypericin
31 Lagerstroemia speciosa Corosolic acid
32 Mallotus philippensis Rottlerin
33 Mangifera indica Mangiferin
34 Mucuna pruriens L-Dopa
35 Ocimum tenuiflorum Rosemarinic acid & Ursolic acid
36 Peganum harmala Harmine & Harmaline
37 Phyllanthus amarus Phyllanthin and Hypophyllanthin
38 Plumbago zeylanica Plumbagin
By GC
39 Crataeva nurvala Lupeol
40 Hedychium spicatum Para methoxy ethyl cinnamate
41 Ocimum Tenuiflorum Ursolic and Oleanolic acid
42 Valeriania officinalis Valerenic acid
By HPTLC
43 Asparagus racemosus Shatavarin-I and Shatavarin-IV
44 Trigonella graceum 4-Hydroyisoleucine
# #
1 Solubility 27 Acid Value
2 Ash 28 Saponification value
3 Acid Insoluble ash 29 Peroxide value
4 pH 30 Free fatty acids
5 Bulk Density 31 Hydroxyl value
6 Aristolochia indica 32 Ester value
7 Tapped Bulk Density 33 Acetyl value
8 Total Soluble Solids 34 Unsapamisiable value
9 Average Weight 35 Iodine Value
10 Uniformity of Weight 36 Friability test
11 Average Fill 37 Total Foreign matter
12 Variation from Average Fill 38 Total Dissolved Solids
13 Disintegration Test 39 Ether Soluble Extractive(Fixed Oil)
14 Hardness Test 40 Crude Fibre
15 Specific Gravity 41 Protein / Nitrogen Estimation
16 Weight / ml 42 Pressure Test in Aerosols
17 Acidity 43 Leakage Test in Aerosols
18 Loss on Drying at 105°C 44 Peroxide value
19 Spreadability 45 Sedimentation Test in Aerosols
20 Clarity of Solution 46 Optical Rotation
21 Consistency 47 Specific Optical Rotation
22 Melting Range 48 Determination of Cineole
23 Boiling Range 49 Loss on ignition
24 Congealing Temperature 50 Sulphated ash
25 Refractive index 51 Particle Size
26 Viscosity By Brookfield
# Solvent Analysis by GC Head Space
1 Methanol
2 Ethanol
3 Acetone
4 Hexane
5 Ethylacetate
6 EDC
7 n-butanol
8 Toluene
9 Tetrahydrofuran
10 Dichloromethane
# Polycyclic Aromatic Hydrocarbons (PAHs)
1 benz[a]anthracene
2 benzo[a]pyrene
# Heavy metal analysis
1 Lead
2 Mercury
3 Arsenic
4 Cadmium
# Mycotoxins
1 Aflatoxins (B1 + B2 + G1 + G2)
2 Ochrotoxins (A & B)
# Naturally Occuring Toxins Test
1 Aristolocholic acid
2 Hypericin
3 Harmal alkaloids
4 Coumarin
5 Methyl eugenol
# Other metals
1 Zinc
2 Copper
3 Aluminium
4 Chromium
5 Cobalt
6 Copper
7 Iron
8 Manganese
9 Barium
10 Tin
11 Nickle
12 Beryllium
13 Vanadium
14 Molybdenum

A wide variety of diseases and dermatological problems are caused by infectious agents. Bacteria plays an important role in the etiology of variety of infectious diseases. Discovery of novel and potent antimicrobial substances help in the management of infectious conditions.


Minimum Inhibitory Concentration (MIC):

The MIC test determines antimicrobial activity of a material against a specific bacteria. The lowest concentration of the test agent that allows no growth/ no more than one or two CFU/ only a slight haze to grow is the MIC. The minimal bactericidal concentration (MBC) or the minimum lethal concentration (MLC) is defined as the lowest concentration of the test agent that will kill a test organism, MLC cannot be done without testing for MIC.


Zone of Inhibition:

This method is used to demonstrate activity / potency of antimicrobials or antibiotics /plant materials by measuring the zone of inhibition against specified microorganisms. This method is very useful for screening of large number of test agents for their antimicrobial activities. The test agents showing good antimicrobial activity can then be taken for MIC studies.


Bioburden Testing:

Bioburden testing will provide an estimate of the total number of bacteria, fungus, etc. present in your sample. Bioburden testing on the products and other ingredients used in the pharmaceutical /food/nutraceutical industry provide control of microbial levels. Bioburden testing determines the number of viable microorganisms on a product.


Environmental Monitoring (Surface testing, equipment testing, air monitoring, glove print)*

The Environmental (Microbial) monitoring programs for controlled environments should assess the effectiveness of the cleaning and sanitation practices by and of the personnel that could have an impact on the bioburden of the controlled environment. Microbial monitoring, regardless of how sophisticated the system may be, will not and need not identify and quantitative all microbial contaminants present in controlled environments. Routine microbiological monitoring should provide sufficient information to ascertain that the controlled environment is operating within an adequate state of control.

The Environmental monitoring program includes an appropriate level of identification of the flora obtained from sampling. A knowledge of the normal flora in controlled environments aids in determining the usual microbial flora anticipated for the facility being monitored, evaluating the effectiveness of the cleaning and sanitation procedures, methods and agents and recovery methods.


Water analysis*

Bacteriological water analysis is a method of analyzing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. It is then possible to draw inferences about the suitability of the water for use from these concentrations. This process is used, for example, to routinely confirm that water is safe for human consumption or that bathing and recreational waters are safe to use.

The interpretation and the action trigger levels for different waters vary depending on the use made of the water. Very stringent levels applying to drinking water whilst more relaxed levels apply to marine bathing waters where much lower volumes of water are expected to be ingested by users.

  • Total Aerobic count
  • Yeast & Mould Count (TFC)
  • Enterobacteriaceae
  • Coliforms
  • E.coli
  • Salmonella
  • Stapylococcus aureus
  • Pseudomonas aeruginosa

Note: *Services available within Bangalore

Outline

Life-science industry is all set to take the centre plunge in the coming years. However one of the major challenges faced by this industry is the lack of skilled manpower which doesn’t match up to the growth curve of industries. There is an increasing demand for, nurturing manpower development in this field.

The current pool of scientific manpower engaged in the biotech industry estimated by Biospectrum magazine is found to be 15,000 and this is expected to touch a level of 60,000 by 2010.

Looking at the increasing demand for trained manpower, Natural Remedies is initiating a unique job-oriented training programme, which will help in orienting the students to adapt to the needs of industry. This comprehensive training programme is a platform for students to augment their practical skills along with an opportunity to work in an herbal drug manufacturing industry.

Who can Apply?

This course is designed for students and fresher’s who would like to have a technical exposure, which suits the needs of fast growing industry.
The training will also be of interest to industry personnel who would like to continue the learning process by increasing their proficiency in new skills. You can select individual course modules best suited to complement your experience or qualification.

Learning Objectivers

Upon completion of this training, attendees will have a clear understanding of the fundamental concepts of research along with background knowledge of pre clinical drug discovery, in vitro screening, operation of basic instruments, writing protocols, assay designing, and checking assay reliability. The most important gain for the attendees is the enhancement of their practical skills which is at par with the industrial standards.

Training programme modules


One Week Training Module

Section I: Overview of the essentials of research

This section gives an insight into the proper preparation and storage of buffers and reagents. Most laboratories lack formal programs to train students on proper technique of pipetting. This section also helps in developing operator pipetting proficiency and consistency in single-channel and multi-channel pipetting, to minimize pipetting errors.

Contents

  • Instrument Handling
  • Reagent and buffer preparation
  • Pipetting skills and calibration

Section II: In vitro screening

Pre-clinical drug development is a stage of research that begins before the clinical trials (testing in humans). One important aspect of Pre clinical drug discovery is in vitro screening of drug molecules which helps in determining the effect of drug molecules on a particular disease target. In vitro screening is critical for the development of biologics and biopharmaceuticals.

The section deals with in vitro target based screening of drug molecules. The screening assays are based on spectrophotometry principles.

Contents

  • Operation, working principle and handling of Microplate reader
  • Biossays in Kinetic and End point mode using Microplate reader
  • Standardization of in vitro assays.
  • Screening of herbal extracts in in vitro assays.
  • Identification of Hits.
  • Data interpretation through sophisticated software programmes.

Students interested to pursue this training programme can contact us at training@naturalremedy.com along with their profile. Any query in this regard will be entertained

Two Week Training Module

Section I: Overview of the essentials of research

This section gives an insight into the proper preparation and storage of buffers and reagents. Most laboratories lack formal programs to train students on proper technique of pipetting. This section also helps in developing operator pipetting proficiency and consistency in single-channel and multi-channel pipetting, to minimize pipetting errors.

Contents

  • Instrument Handling
  • Reagent and buffer preparation
  • Pipetting skills and calibration

Section II: In vitro screening with different platforms

In vitro screening involves testing the drug candidates on various targets. The screening involves target based assay development. The assays are based on various platforms or assay formats.

This course is designed to provide an understanding of target based drug screening based on two different platforms and the associated instruments.

Spectrophotometry:

  • Operation, working principle and handling of Microplate reader
  • Biossays in Kinetic and End point mode using Microplate reader
  • Standardization of in vitro assays.
  • Screening of herbal extracts in in vitro assays.
  • Identification of Hits.
  • Data interpretation through sophisticated software programmes.

Spectrofluorometry:

  • Operation, working principle and handling of Spectrofluorimeter (Fluostar) and the associated software programme.
  • Biossays in Kinetic and End point mode using FLUOstar.
  • Bioassay development and validation using assay performance measures.
  • Screening inhibitors using different bioassay models
  • Bioassay miniaturization
  • Data interpretation through sophisticated software programmes

Application Procedure

Students interested to pursue this training programme can contact us at training@naturalremedy.com along with their profile. Any query in this regard will be entertained.

One Month Training Module

Section I: Overview of the essentials of research

This section gives an insight into the proper preparation and storage of buffers and reagents. Most laboratories lack formal programs to train students on proper technique of pipetting. This section also helps in developing operator pipetting proficiency and consistency in single-channel and multi-channel pipetting, to minimize pipetting errors.

Contents

  • Instrument Handling
  • Reagent and buffer preparation
  • Pipetting skills and calibration

Section II: In vitro screening and Bio-analytical techniques in drug discovery

This module provides a preamble to the basics of Bioanalytical instrumentation and associated techniques which play an important role in quality control, pharmacokinetics and even pharmacodynamics of drug candidates along with the details of target based drug screening based on two different platforms and the associated instrumentation.

Spectrophotometry:

  • Operation, working principle and handling of Microplate reader
  • Biossays in Kinetic and End point mode using Microplate reader
  • Standardization of in vitro assays.
  • Screening of herbal extracts in in vitro assays.
  • Identification of Hits.
  • Data interpretation through sophisticated software programmes.

Spectrofluorometry:

  • Operation, working principle and handling of Spectrofluorimeter (Fluostar) and the associated software programme
  • Biossays in Kinetic and End point mode using FLUOstar
  • Bioassay development and validation using assay performance measures
  • Screening inhibitors using different bioassay models
  • Bioassay miniaturization
  • Data interpretation through sophisticated software programmes
  • Bio-analytical techniques
  • Thin layer chromatography (TLC)
  • High performance liquid chromatography (HPLC)

Application Procedure

Students interested to pursue this training programme can contact us at training@naturalremedy.com along with their profile. Any query in this regard will be entertained.

We are a leading group in the field of phytochemistry in India. Our dedicated team of phytochemists is involved in isolating bioactive phytochemicals from different medicinal plants. We have been supplying phytochemical reference substances over a decade now. Our customers include herbal companies, national laboratories, Pharmacopoeia bodies, universities and other governmental and non-governmental organizations involved in herbal research.

Currently, we offer more than 200 phytochemical reference substances and we are in the process of isolating many more phytochemicals from different medicinal plants. Our strength lies in quality and reliability of our products and services. Our passion for phytocompounds is driving us to do more in this area and we solicit your active participation in this endeavour. For more details please visit www.phytocompounds.com

Standardized botanical powders for nutraceutical, food and beverage industries.

* Tested for pesticides, heavy metals, mycotoxins & microbial contaminants, to comply with USP limits, in ISO 17025 certified in-house laboratory.

Products

Standardized Botanical Powders Common Name Part Used Standardized with reference to
Andrographis paniculata* Andrographis, Creat, Kalmegh Aerial part Andrographolide >0.5% w/w
Bacopa monnieri* Bacopa, Brahmi Aerial part Triterpene glycosides (sum of bacopaside I and bacoside A) > 1.5% w/w
Centella asiatica* Gotu kola, Mandukaparni Aerial part Triterpene derivatives (sum of madeccassoside, asiaticoside, madecassic acid, Asiatic acid) > 0.75% w/w
Cuminum cyminum Cumin, Jeera Fruit Volatile oil > 0.5% v/w
Curcuma longa Turmeric, Haridra Rhizome Curcuminoids > 1.5% w/w
Volatile oil > 1.5% v/w
Glycyrrhiza glabra Licorice, Yastimadhu Root Glycyrrhizinic acid > 1.5% w/w
Medicago sativa Alfalfa Aerial part
Moringa oleifera* Horseradish tree, Moringa Leaf Protein > 15% w/w
Ocimum tenuiflorum* Holy basil, Tulasi Aerial part Triterpenes (sum of oleanolic and ursolic acid) > 0.2% w/w
Piper longum* Long pepper, Pippali Fruit Piperine > 0.25% w/w
Piper nigrum Pepper, Maricha Fruit Piperine > 1.0% w/w
Tribulus terrestris Tribulus, Puncture vine Fruit Total saponins > 1.5% w/w
Trigonella foenum-graceum Fenugreek, Methi Seed Total saponins > 5.0% w/w
Withania somnifera* Ashwagandha, Winter cherry Root Total withanolides (sum of withanolide aglycones & withanolide glycosides) > 0.1% w/w
Zingiber officinale Ginger, Shunthi Rhizome Total gingerols (sum of 6 gingerol, 8 gingerol & 10 gingerol) > 0.25% w/w
Cinnamomum verum Cinnamon Bark Total polyphenols > 2.5% w/w, Cinnamaldehyde > 0.3% w/w
Gymnema sylvestre Gymnema Leaf Total gymnemic acid > 1.0% w/w
Momordica charantia Bitter melon Fruit Total bitters >5.0% w/w
Tinospora cordifolia Giloy Stem
Urtica dioica Nettle Leaf / Root Scopoletin > 3.0% w/w

Available grades:

  • Regular
  • Premium (Organic, Customized, Low Lead, etc)

Features:

  • • Certified botanical identity
  • • 100 % Natural
  • • Steam sterilized
  • • Available mesh size 40 & 60

* Under supervised cultivation